B.G. HAN, R. WALTON, A. SONG, P. HWU, M. STUBBS, S. YANNONE, P. ARBELAEZ, M. DONG AND R. GLAESER
JOURNAL OF STRUCTURAL BIOLOGY, 2012
Chemical biotinylation of protein complexes followed by binding to two-dimensional (monolayer) crystals of streptavidin is shown to be an effective way to prepare cryo-EM specimens from samples at low protein concentration. Three different multiprotein complexes are used to demonstrate the generality of this method. In addition, native thermosomes, purified from Sulfolobus solfataricus P2, are used to demonstrate that a uniform distribution of Euler angles is produced, even though this particle is known to adopt a preferred orientation when other methods of cryo-EM specimen preparation are used.
Addrs. Cra. 1 E No. 19A - 40. Mario Laserna Building - School of Engineering, Bogotá, Colombia, Zip 111711, Ph. +(571) 332 4327, 332 4328, 332 4329
Universidad de los Andes | Monitored by Mineducación
Recognition as University: Decree 1297 of May 30th, 1964.
Recognition as legal entity: Resolution 28 of February 23, 1949 Minjusticia.
© Universidad de los Andes. All rights reserved.